2×Hieff™ PCR Master Mix contains Hieff™ Taq DNA Polymerase (Cat#10101), dNTPs, and other PCR-required components. The Master Mix is stable for 3 months at 4°C with our customized stabilizers. The pre-mix solution is optimized for conventional PCR and ready to use by adding DNA template and primers. The PCR products can be loaded directly for electrophoresis with pre-loaded bromophenol blue dye. The amplified products contain 3 '-dA protrusion and can be easily cloned into T vector. The 2×Hieff™ PCR Master Mix simplifies PCR procedure and reduces contamination. Features Convenient, ready-to-use mix Thermostable—half-life is more than 40 min at 95°C Generates PCR products with 3'-dA overhangs Applications Genetyping Routine PCR amplification of DNA fragments High throughput PCR DNA labeling Specifications Fidelity (vs. Taq) 1 × Hot Start No Overhang 3'-A Polymerase Taq DNA Polymerase Reaction Format SuperMix or Master Mix Reaction Speed Standard Product Type PCR Master Mix (2×) Components Components No. Name 10102ES03 10102ES08 10102ES50 10102ES60 10102 2×Hieff™ PCR Master Mix (With Dye) 1 mL 5×1 mL 50×1 mL 100×1 mL Figures Figure1. The expected 1.2 kb PCR products can be amplified with 2×Hieff PCR Master Mix. The Master Mix was stored at -20°C for 1 year following another 3 months at 4°C and 1 month at 25°C. Template: Arabidopsis genome. Annealing temperature: 60°C. Extension time: 40 sec.
