Deoxyribonuclease I (DNase I) Bovine pancreas _ 10607ES

Yeasen - Leading Innovation in Molecular Enzymes and Reagents Verified independent store

→

Deoxyribonuclease I (DNase I) is a nuclease found in a wide variety of cells and tissues. It targets and cleaves the phosphodiester bond adjacent to the pyrimidine to produce polyn ucleotides with a phosphate group at the 5' end and a hydroxyl group at the 3' end. The average digestion product is about the size of a polynucleotide. DNase can catalyse different forms of DNA such as single-stranded DNA, double-stranded DNA and even chromatin (its cutting rate is affected by histones). The optimum operating range is pH 7-8. The activity of DNase is Ca2+ dependent and can be activated by divalent metal ions such as Co2+, Mn2+ and Zn2+. 5 mM Ca2+ can protect the enzyme from hydrolysis. In the presence of Mg2+, the enzyme can randomly recognise and cut any site on either DNA chain; in the presence of Mn2+, it can recognise both DNA chains simultaneously and cut at almost the same site. DNase I was first isolated from pancreas, and mammalian pancreas is still a major source of the enzyme. This product is derived from bovine pancreas and consists of four chromatographically distinct components A, B, C and D in a molar ratio of 4:1:1, with component D being very small. It is often used in molecular biology experiments to remove DNA from proteins or to create gaps in DNA to allow the insertion of labelled bases into DNA. This product is supplied as a lyophilised powder containing calcium chloride with an enzyme activity of ≥2000 Kunitz units/mg protein. Features High activity Low residual RNase Stable Quality: No variations between batches Applications DNA removal in RNA extraction; DNA removal in in vitro transcription; DNase I blotting; nick translation; RNA purification; Specification CAS NO. 9003-98-9 Molecular Weight ~31 kDa Kunitz Units ≥2000 Kunitz Units/mg protein Type Type IV Optimal pH 7~8 Appearance White to light brown freeze-dried powder Purity Protein: ≥80% by Biuret Solubility 0.15 M NaCl：5 mg/mL, colorless and transparent solution Activators Various divalent metal ions such as Mg2+、Mn2+, Ca2+, Co2+, and Zn2+ Inhibitors β-Mercaptoethanol; Chelating agent; SDS; Actin Unit Definition Under the conditions of 25°C and pH 5.0, DNase catalyses the DNA, and the change in ΔA260 per ml per minute increase of 0.001 is defined as one enzyme activity unit (Kunitz unit). Components Category Components No. Name 10607ES15 10607ES81 Part I 10607-A DNase I 15 KU 10 × 15 KU Part II 10607-B DNase I Storage Buffer 12 mL 120 mL 10607-C 1 M MgCl2 12 mL 120 mL Show more