GTP, Guanosine-5'-triphosphate, can be used in various of molecular biology applications, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. In addition, GTP plays an important role in signal transduction as a phosphate or pyrophosphate messenger: GTP can activate G proteins, which can induce multiple protein kinase mediated cascade reactions, causing a variety of cytological behaviors, such as cell proliferation, differentiation, etc. GTP can also be used as a high-energy precursor of single nucleotides to participate in the synthesis of DNase and RNase.This product is a transparent colorless aqueous solution prepared with GTP tris solution, and free of DNase and RNase contamination.This product is produced in accordance with GMP process requirements and provided in liquid form. Feature Validated, product-specific processes and analytical methods Product-specific stability Documentation follows applicable GMP guidelines AOF production process and raw materials (TSE & BSE) Nitrosamine statement Regulatory support documents available Large-scale production The increased IVT yield and the decreased dsRNA content under the optimized Tris NTP reaction system Application RNA synthesis and amplification Building block for in vitro transcription Specification CAS No 86-01-1 (free acid) Formula C10H16N5O14P3 (free acid) Molecular weight 523.18 g/moL (free acid) Purity(HPLC) ≥ 99% Content 100 mM ± 3 mM Structure Component Components No. Name 10655ES03 10655ES10 10655ES60 10655 GTP Tris Solution GMP-grade (100 mM) 1 mL 10 mL 100 mL Figures Increased the IVT yield Figure 1. The IVT yield was significantly increased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system. Decreased the dsRNA content Figure 2. The content of dsRNA was significantly decreased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system. The content of dsRNA was detected by the Dot Blot method.
