This product is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GCTCTTCN1/N4-3'. Use to digest plasmids to prepare poly (A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends. This product is produced in accordance with GMP process requirements and provided in a liquid form. Features DA DMF Certified** (MF038306) GMP Grade, Animal-Origin Free: Manufactured under ISO 13485 and GMP standards High Efficiency, Low Star Activity: Full digestion at 37°C in 16 hours, no star activity Nuclease-Free: Rigorously tested and confirmed free of endonucleases, exonucleases, and RNases to ensure sample integrity and reliable downstream results Performance Matches Leading Brands Application Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G); Linearize plasmid template before in vitro transcription Restriction digest Specification Expression Host Recombinant E. coli with BspQI gene Reaction Temperature 50℃ Storage Buffer 20 mM Tris-HCl, 0.5 M KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% Glycerol Unit Definition 1 unit: The amount of enzyme required to digest 1 μg of λDNA within 1 h at 50℃ in a 50 μL system Components Components No. Name 10664ES76(500 U) 10664ES86(2,500 U) 10664ES92(10 KU) 10664ES98(100 KU) 10664 BspQI GMP-grade (10 U/μL) 50 μL 250 μL 1 mL 10 mL Figures 1. Enhanced Restriction Enzyme Digestion Efficiency Figure 1. Comparison of Digestion Efficiency at Different Enzyme Amounts (0.125–4 U): 2 U Delivers Optimal Performance In a 50 μL reaction system, 1 μg of λDNA was treated with the corresponding amount of BspQI (incubate at 50 °C for 60 min and then incubate at 80 °C for 20 min to inactivate BspQI). Then 20 μL of the reaction solution was loaded.M: DNA markerC: The control group without BspQI treatment 2. High-Efficiency Enzymatic Digestion in 0.5–3 Hours Figure 2. Digestion Performance Across Incubation Times (0.1–3 h): Consistent Results with NEB.
