UDG (uracil DNA glycosylase) can catalyze the hydrolysis of the N-glycosidic link between the uracil base and the sugar-phosphate backbone in ssDNA and dsDNA. It can easily control aerosol pollution and is suitable for common molecular biology systems such as PCR, qPCR, RT-qPCR and LAMP. Compared to regular UDG, Heat-Labile UDG avoids the potential residual activity that regular UDG may have after inactivation, which could degrade dU-containing amplification products at room temperature. Applications Remove aerosol contamination from PCR products containing dU.Remove single-stranded or double-stranded DNA uracil bases. Specifications Unit Definition The amount of enzyme required to completely degrade 1 μg of dsDNA containing uracil within 30 minutes at 25°C is defined as 1 activity unit (U). Deactivation method 94℃, 2-5 minutes. Components No. Name 10707ES60 (100 U) 10707ES76 (500 U) 10707ES90 (5 KU) 10707ES92 (10 KU) 10707 Uracil DNA Glycosylase (UDG, Glyceol-free), (1 U/μL) 100 μL 500 μL 5 mL 10 mL Precautions For your safety and health, please wear a lab coat and disposable gloves. Thermosensitive UDG is active in most PCR reaction buffers.
