Hieff UNICON™ HotStart Taq DNA Polymerase is a mixture of Hieff UNICON™ Taq Antibody(Catalog #31301ES)and Hieff™ Taq DNA Polymerase. Hieff UNICON™ Taq Antibody had a high affinity for Hieff™ Taq DNA Polymerase, and the activity of Hieff™ Taq DNA Polymerase could still be blocked after being treated at 50℃ for 30 min. The product was completely inactivated by heating at pre denaturation temperature for 30 seconds, releasing DNA polymerase activity. The hot start Taq enzyme can effectively inhibit the amplification caused by non-specific annealing of primers. Hieff UNICON™ HotStart Taq DNA Polymerase is suitable for hot start PCR and qPCR. Hieff™ Taq DNA Polymerase in this product is a heat stable recombinant DNA polymerase with high template affinity, which is very suitable for amplification of low copy template. The amplified product has 3 '- dA and can be easily cloned into T vector. Features Closed Detection of DNA Polymerase Activity：In the Hieff UNICON™ HotStart Taq Buffer (Mg2+ Plus) ，the activity release was less than 5% after incubation at 65℃ for 30 min. DNA Polymerase Activity Release Detection ：In the HieffUNICON™ HotStart Taq Buffer (Mg2+ Plus) ，the activity release was higher than 95% when heated at 95℃ for 30 sec. Exonuclease Activity: In 20 μL reaction system, 10 U of this product and 0.6 μg of λ - HindIII were incubated at 74℃ for 1 h, and the electrophoresis band of DNA had no change. Endonuclease Activity: In 20 μL reaction system, 10 U of the product and 0.6 μg of supercoiled pBR322 DNA were incubated at 74 ℃ for 1 h, and the DNA electrophoretic bands did not change. Residual E.coli gDNA Test: The E.coil 16S rDNA gene was amplified by adding 2 U of the product into 50 μL system and using sterile ddH2O as template. After 35 cycles, the amplified products were subjected to 1% agarose gel electrophoresis and EB staining without amplification bands. Specifications Polymerase Taq DNA Polymerase Purity ≥ 95%（SDS-PAGE） Hot Start Built-In Hot Start Reaction Speed Standard Exonuclease Activity 5' - 3' Unit Definition Using the activated DNA of salmon sperm as template / primer, the activity was defined as one active unit (U) when 10 nmol of total nucleotide was ingested as acid insoluble substance at 74℃ for 30 min. Components Name 10730ES00 (500 U) 10730ES01 (5,000 U) 10730ES03 (50,000 U) 10730ES25 (1,250,000 U) HotStart Taq DNA Polymerase (50 U/μL) 10 μL 100 μL 1 mL 25 mL
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