Hifair™ V Reverse Transcriptase (600U/μL, Glycerol-free) -11301ES

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Hifair™ V Reverse Transcriptase is an updated version of Hieff™ M-MLV (H-) Reverse Transcriptase obtained by genetic engineering technology. It has higher cDNA synthesis ability, t hermal stability, and reaction temperature limit (up to 60°C) than Hieff™ M-MLV (H-) Reverse Transcriptase. The synthesized cDNA product is up to 10kb. Hifair™ V Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex secondary structures or low-copy genes. Features Outstanding thermal resistance. Suitable for RT-PCR/RT-qPCR applications. Glycerol-free, more suitable for lyophilization. Specifications Cat.No. 11301ES06 / 11301ES12 / 11301ES62/ 11301ES75 /11301ES96 Size 3 KU / 12 KU / 120 KU / 300 KU / 3000 KU Unit Definition One unit (U) is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into an acid-insoluble substance in 10 minutes at 37℃, using Poly(A)·Oligo(dT) as the template-primer. Components Components Name 11301ES06 11301ES12 11301ES62 11301ES75 11301ES96 Hifair™ V Reverse Transcriptase (600 U/μL) 5 μL 20 μL 200 μL 500 μL 5 mL Performance demonstration 1. Capable of withstanding a reaction temperature of 58°C, it is suitable for the reverse transcription of complex RNA templates. Figure 1. The Reverse Transcriptase of Hifair™ V Reverse Transcriptase and the brand T* were incubated at 55℃ and 58℃ respectively for 30min to detect the residual enzyme activity. The results showed that Hifair™ V Reverse Transcriptase could still maintain 70% activity after incubation at 58℃ for 30min, which was much higher than that of the brand T*. 2. The detection rate of RT-qPCR was better than that of other brands, and the detection range was 1 pg-1 μg. Figure 2. Using 1 pg-1 μg (7 gradients) total RNA of 293T cells as template, reverse transcription was performed using Hifair™ V one-step RT-gDNA digestion SuperMix for qPCR(Cat#11142), V*, T*. The obtained cDNA was quantitatively analyzed by qPCR. The results showed that the sensitivity of 11142ES could reach 1 pg, which was better than that of other brands. Show more