Firefly luciferase is a protein with a molecular weight of approximately 61 kDa. In the presence of ATP, magnesium ions, and oxygen, it can catalyze the oxidation of luciferin to oxyluciferin, during which a bioluminescence with a wavelength of around 560 nm is emitted. Detection principle as shown in the figure: Figure 1: Schematic diagram of the firefly luciferase detection principle YEASEN Biotech's Bio-One Step Luciferase Reporter Gene Detection Kit is characterized by its ease of operation and high signal strength. There is no need to wash or collect cells before the experiment; simply add the reagent directly to the culture medium, and the lysis and detection of cells are completed in one step, saving experimental time. This kit achieves a relative balance between signal strength and stability. It maintains a relatively high signal strength while keeping the signal intensity relatively stable over a certain period, ensuring that the experimental results are accurate and reliable. The half-life typically reaches over 1 hour. The product has a wide linear range for sample determination. In a 96-well plate, it can exhibit a good linear relationship within a range of 200,000 cells. Features Long signal half-life: The signal half-life is 1 hour, meeting the needs of high-throughput experiments. Strong fluorescence signal: The signal value can reach 8 orders of magnitude, with a large detection window. Convenient operation: One-step procedure, avoiding complicated experimental processes. Applications Signaling Pathway Research Candidate Drug Screening T Cell Killing Assay Specifications Form liquid Luciferase Firefly Signal Half-life 1 h Number of Product Components 1 Workflow One Step Components Components No. Name 11411ES60 11411ES80 11411ES81 11411 Bio-One Step Luciferase Assay Kit 10 mL 10×10 mL 100 mL Figures High signal values Figure 1. YEASEN Bio-One Step has better stability and higher signal values compared to its competitors. Good stability Figure 2. Five freeze-thaw cycles have virtually no impact on the signal intensity of the Yeasen Bio-One Step product. Wide ADCC functional assay window Figure 3. Bio-One Step for CLDN18.2 ADCC detection: Assays performed using this method exhibit high signal intensity, low background, and a distinct signal window, meeting the requirements for functional validation.
