Hieff NGS™ FFPE DNA Repair Reagent is specifically designed for FFPE samples to repair varying degrees of damage in low-quality FFPE DNA, enhancing library yield and sequencing quality. This product can be directly used with Yeasen Hieff NGS™ Library Preparation Kits (Cat#12197/Cat#12927) and the A-tailing module (Cat# 12608), with compatible systems that can repair 5 ng to 1 μg of fragmented FFPE DNA. Additionally, this product is also suitable for use with Yeasen Fragmentation Enzyme-based Library Preparation Kit. Features High Repair Efficiency: Provides higher repair efficiency for FFPE DNA.Streamlined Workflow: Simple operation with strong adaptability.High Compatibility: Enzyme components can be used with the end-repair and A-tailing system of library preparation kits, requiring only a single buffer to complete FFPE DNA repair and end-repair/dA-tailing. Applications Repair of damaged low-quality FFPE samples Library preparation for low-quality FFPE samples Specifications Cat.No. 12606ES24/12606ES96 Size 24 T/96 T Components Components No. Name 12606ES24 12606ES96 12606-A FFPE DNA Repair Mix 96 μL 384 μL 12606-B Endprep Buffer 2.0 144 μL 576 μL Figures Figure1. The impact of FFPE repair reagents on library yield During the library construction process, two sets of experiments were conducted to compare the same samples. One group included a repair reagent in the end-repair and A-tailing step, while the other group did not. The experimental results showed that the FFPE repair reagent significantly increased library yield for low-quality FFPE samples by repairing the samples, whereas its effect on high-quality FFPE samples was less pronounced.
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