Hieff NGS™ mRNA Isolation Master Kit V2 is an upgraded magnetic bead kit specifically designed for purifying mRNA. The mRNA Capture beads are micron-sized paramagnetic microspheres conjugated with Oligo (dT). They combine with the mRNA carrying a poly (A) tail to separate and purify the mRNA from 10 ng to 4 μg of total RNA with good integrity. Features Wide compatibility: Compatible with total RNA input amounts ranging from 10 ng to 4 μg, and compatible with total RNA library preparation kits from different brands Inhibitor tolerance: High mRNA yield and low rRNA residual for samples containing inhibitors, such as herbal medicines, polysaccharide- and polyphenol-rich samples, and tumor tissues. High mRNA purity: Suitable for eukaryotic samples with poly(A) tails. Specifications Product Line Magnetic Bead Starting Material RNA(10 ng - 4 μg) mRNA isolation method Oligo dT magnetic beads For Use With (Application) RNA enrichment Components Components No. Name 12629ES08 12629ES24 12629ES96 12629ES97 12629ES98 12629-A mRNA Capture Beads 2.0 400 μL 1.2 mL 4.8 mL 5.5 mL 24 mL 12629-B Beads Binding Buffer 2.0 400 μL 1.2 mL 4.8 mL 5.5 mL 24 mL 12629-C Beads Wash Buffer 2.0 5 mL 15 mL 60 mL 60 mL 240 mL 12629-D Tris Buffer 2.0 400 mL 1.2 mL 4.8 mL 5.5 mL 24 mL 12629-E Nuclease-free water 1 mL 1 mL 4 mL 4 mL 20 mL Figures 1.Workflow Figure 1. mRNA isolation workflow 2.Wide input range compatibility Figure 2. rRNA Residual Levels Across Different Input Amounts Library preparation was performed using 12629 and RNA Library Preparation Kit (Cat#12308, with both strand-specific and non-strand-specific protocols) on conventional RNA samples with varying input amounts. The rRNA residual rate was significantly lower compared to Supplier V*. 3.rRNA Removal Across Compatible Species Figure 3. rRNA Residual Levels Across Different Species Library preparation was performed using the 12629 and RNA Library Preparation Kit (Cat#12308, strand-specific protocol). The rRNA residual rate was significantly lower compared to Supplier B*. 4.Stability Figure 4. Stability Test 12629 stored at 4°C for 2 weeks and 37°C for 2 weeks, combined with RNA library preparation kit(Cat#12308), shows consistent library yield and rRNA depletion using 1 µg of 293 RNA for strand-specific library preparation.
