This product is suitable for multiple RT-PCR experiments, and it is suitable for co-detection of DNA&RNA pathogenic microorganisms.This kit is based on super multiplex RT-PCR, combined with hot start modification technology and matched with the optimal buffer system, which can realize the re-amplification of Panel with panel multiplicity of 1-2,000. This product is compatible with reverse transcription system, and the GC content of amplicon can cover the range of 25-75%. It has the characteristics of high uniformity, high specificity and high sensitivity. This reagent is suitable for multiple amplification of fragments with different lengths, such as 100-500 bp, and can also be used for the capture of amplicon more than thousand. Features Simplify operation: packing the pre-mixed solution , and only need to add templates and primers in the experiment. RT-PCR is combined into one step. Widely compatibility: compatible with microbial research, pathogen detection, genetic disease detection and agricultural detection etc. Excellent amplification performance: high detection rate, excellent specificity and good uniformity. Strict control of background bacteria: clean workshop, professional personnel and strict quality control. Applications tNGS；Microbial detection；Multiplex PCR；RT-PCR Specifications Cat.No. 12950ES24/ 12950ES48 / 12950ES96 Size 24 T / 48 T / 96 T Components Components No. Name 12950ES24 12950ES48 12950ES96 12950ES98 12950-A RT-PCR buffer 168 μL 336 μL 672 μL 7 mL 12950-B RT-PCR Enzyme mix 12 μL 24 μL 48 μL 500 μL 12950-C 4X PCR mix 180 μL 360 μL 720 μL 7.5 mL Figures Figure 1. Detection of microorganisms DNA &RNA Libraries were prepared using Cat#12950 protocol ,To evaluate the detection of DNA and RNA species with 100 copies/T RNA Virus Standard and 10 pg the ZymoBIOMICS Microbial Community DNA Standard (Zymo Research #D6306). The results show that the performance of 12950 is better than other suppliers.
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