Hieff™ LongSeq Plasmid Fragmentation and Ligation Module _ 13305ES

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Hieff™ LongSeq Plasmid Fragmentation and Ligation Module is a dedicated enzymatic module developed for third-generation Nanopore sequencing platforms. When used in combination with Cat. No. 13304ES, it enables streamlined plasmid library construction. This kit is optimized specifically for plasmid DNA library preparation. Following enzymatic fragmentation, end repair, and dA-tailing, no purification step is required—samples can proceed directly to barcode ligation, significantly simplifying the workflow. Features Wide input range: 50 ng – 1 µg plasmid DNA Fast workflow: ~2 hours from DNA to library Enzyme-based fragmentation: controlled, low-damage digestion Long-read friendly: preserves plasmid integrity and long inserts Consistent performance: strict batch-to-batch quality control Components Components NO. Name 13305ES08 13305ES24 13305ES96 8 T 24 T 96 T 13305-A Smearase™ Buffer 80 μL 240 μL 960 μL 13305-B Smearase™ Enzyme 40 μL 120 μL 480 μL 13305-C Rapid Ligase Master Mix 280 μL 840 μL 4×840 μL Figure Excellent Nanopore sequencing performance Sequencing Size Distribution Figure 1. High-Quality Nanopore Sequencing of Plasmid Libraries Prepared Using Yeasen Enzymatic Fragmentation(13305ES+13304ES). Plasmid libraries were prepared from 100–200 ng input DNA through a rapid 2-minute enzymatic fragmentation process. The resulting libraries achieved an N50 of 2–5 kb, showcasing robust and reproducible long-read sequencing performance on Oxford Nanopore platforms. Show more