FuniCut™ series endonucleases are fast restriction enzymes developed through genetic engineering that can precisely cut DNA within 5–15 minutes. These enzymes are suitable for rapid digestion of plasmid DNA, PCR products, genomic DNA, and more. All FuniCut™ fast restriction enzymes share the same digestion buffer, which simplifies reaction setup. Additionally, they have a high enzyme activity margin, making them capable of easily handling excess substrate or challenging templates. Specifications Cat. No. 15003ES76 / 15003ES20 Size 500 T / 20,000 T Recognition Site 5'-G↓GATCC-3' 3'-CCTAG↑G-5' Recommended Reaction Conditions 1× FuniCut™ Buffer; incubate at 37°C Enzyme activity 20 U/μL Inactivation Conditions Not heat-inactivatable. Use phenol-chloroform extraction or column purification. Cohesive-end ligase None Effect of methylation Dam, Dcm, CpG, EcoKI, EcoBI: Not affected Components Components No. Name 15003ES76 15003ES20 15003-A FuniCut™ BamHI 500 μL 20 mL 15003-B 10×FuniCut™ Buffer 3×1 mL 120 mL 15003-C 10×FuniCut™ Color Buffer* 3×1 mL 120 mL 【Note】: 10× FuniCut™ Color Buffer includes red and yellow tracking dyes, allowing direct loading of reaction products onto agarose gels without additional loading buffer. In 1% agarose gel electrophoresis, the red dye co-migrates with ~2500 bp double-stranded DNA fragments, while the yellow dye migrates similarly to ~10 bp double-stranded DNA fragments.
