The FuniCut™ series of restriction endonucleases are rapidly acting, genetically engineered enzymes capable of precisely cutting DNA within 5 to 15 minutes. They are ideal for the rapid digestion of plasmid DNA, PCR products, genomic DNA, and more. The FuniCut™ series shares a universal digestion buffer, simplifying the reaction setup, and offers excellent enzyme activity redundancy, making it easy to handle substrate excess or challenging templates. Specifications Enzyme SpeI Recognition Site 5'-A↓CTAGT-3' 3'-TGATC↑A-5' Recommended Reaction Conditions 1× FuniCut™ Buffer; incubate at 37°C. Enzyme Activity 20 U/μL Inactivation Conditions Incubate at 80°C for 20 minutes. Isoschizomers Ahll, Bcul Components Components No. Name 15028ES50 15028-A FuniCut™ Spel 50 μL 15028-B 10xFuniCut™ Buffer 1 mL 15028-C 10xFuniCut™ Color Buffer* 1 mL [Note]:*10xFuniCut™ Color Buffer includes red and yellow tracking dyes, allowing direct use of the product for gel electrophoresis. The red dye migrates similarly to a 2500 bp double-stranded DNA fragment in 1% agarose gel; the yellow dye migrates similarly to a 10 bp double-stranded DNA fragment in 1% agarose gel.
