Hifair™ Advanced Multiplex One Step RT-qPCR Probe Mix (UDG Plus) is a reagent kit designed for multiplex quantitative PCR (qPCR) using RNA as the template. In this workflow, reverse transcription and qPCR are performed in a single reaction tube, simplifying experimental procedures and reducing the risk of contamination. This kit employs a thermostable Reverse Transcriptase for efficient first-strand cDNA synthesis and HotStart Taq DNA Polymerase for quantitative amplification. The kit includes an optimized MP Buffer and Enzyme Mix. The buffer already contains Mg²⁺ and dNTPs, along with components that effectively suppress non-specific PCR amplification and enhance the efficiency of multiplex qPCR reactions—enabling robust multiplex amplification while maintaining primer amplification efficiency. Additionally, the dUTP/UDG system is incorporated to effectively prevent aerosol-mediated contamination. Features All-in-One Master Mix: Buffers and enzymes precombined in a single tube for one-step RT-qPCR setup — pipette and run. Exceptional Stability: Maintains performance after accelerated aging at 37°C for 7 days and ≥20 freeze–thaw cycles, enabling reliable storage and transport. dUTP/UDG anti-pollution system: Incorporates dUTP/UDG enzyme to prevent amplicon carryover contamination for cleaner results. Components Name 16921ES50 16921ES60 16921ES80 16921ES92 5×RT-qPCR Master Mix 250 μL 500 μL 5 mL 50 mL Application RT-qPCR Detection; Diagnostic pathogen detection (respiratory tract, intestinal tract, etc.); animal pathogen detection (non-swine fever, etc.); food testing (microorganisms, etc.). Figures 1. High sensitivity&Batch-to-Batch Stability Figure 1.Three-Batch Performance Evaluation (Sensitivity and Positive Control Testing) A 10-fold serial dilution of real sample template (Ct 36–40) was tested in eight replicates to assess sensitivity across three production batches. In parallel, two-well amplification of the positive control was performed to evaluate overall amplification performance and batch consistency. 2. Strong stability Figure 2. Thermal accelerated stability testing Two batches of Yeasen 16921 reagents were stored at 37°C for 3 days and compared with reagents kept under standard storage conditions. PRRSV real samples and positive controls were amplified in parallel. The Ct values of both target and positive control remained consistent across conditions, and all samples were successfully detected.
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