The Hieff™ Blood RNA Kit is suitable for the extraction of RNA from samples such as blood, plasma, serum, and lymph fluid. The silica-based material used in the centrifugal adsorption column is a unique new material developed by our company. It, combined with our unique lysis buffer formulation, allows for the maximum recovery of high-purity RNA. The extracted RNA has high purity, stable and reliable quality, and can be used for various downstream applications, such as RT-PCR, RT-qPCR, in vitro transcription, molecular cloning, etc. Features Fast and simple: simple operation, single sample extraction is completed in 30 minutes. Safe and non-toxic: no isopropanol extraction is required. Application Blood samples: suitable for RNA extraction from blood, plasma, serum, lymph and other samples. Components Prat Components No. Name 19241ES50 Part I 19241-A Lysis Buffer / LB Buffer B3 50 mL Part II 19241-B Hieff™ RNA Column B3 50 19241-C 2 mL Collection Tube B3 50 19241-D Deproteinized Solution* / PL Buffer B3* 16 mL 19241-E Wash Buffer* 12 mL 19241-F RNase-free H2O 5 mL Application RNA extraction from blood, plasma, serum, or lymph samples. Figures Whole Blood RNA Extraction Table 1. Concentration and purity QC results of the extracted RNA Sample No. Concentration (ng/μL) OD260/280 OD260/230 Extraction reagents 1 18.24 1.99 0.92 Yeasen-19241 2 18.14 2.01 1.17 3 25.23 1.76 0.60 4 11.57 1.39 0.14 5 13.54 1.47 0.11 Supplier M* 6 14.51 1.49 0.11 Six 250 μL fresh blood samples were extracted using 19241 and M brand kits, with RNA eluted in 50 μL RNase-free water. 1 μL of each eluate was used to measure RNA concentration. Result: 19241 showed higher RNA yield. Figure 1. Blood RNA Integrity QC Results Six 250 μL fresh blood samples were extracted using 19241 and M brand kits, with RNA eluted in 50 μL RNase-free water. 5 μL of each eluate was analyzed for RNA integrity and band intensity.Result: RNA extracted with 19241 showed better integrity and clearer bands.
