Carrier RNA helps to improve the binding and elution efficiency on the purification column during the purification of trace nucleic acids and reduces the likelihood of RNase degradation of trace RNA. The addition of Carrier RNA during nucleic acid purification can significantly improve the recovery efficiency of trace nucleic acids and is an essential component for the purification of trace nucleic acids. The concentration of this product is 6 μg/μL and there is no DNase or RNase enzyme contamination. It is compatible with subsequent conventional molecular biology applications such as viral nucleic acid extraction, trace DNA extraction and subsequent RT-PCR, RT-qPCR and other experiments. Features High purity and stability Broad compatibility: compatible with a variety of sample types (plasma, serum, FFPE tissue, etc.) and extraction kits. Enhanced nucleic acid recovery efficiency Stable Quality: No variations between batches Applications Nucleic acid extraction; Viral nucleic acid extraction; Micro-nucleic acid extraction Specification Cat.No. Size Price 19701ES03 1 mL ＄105 19701ES08 5 mL ＄405 19701ES60 100 mL ＄6505 Components Components No. Name 19701ES03 19701ES08 19701ES60 19701 Carrier RNA, 6 μg/μL 1 mL 5 mL 100 mL Figures A. The addition of carrier RNA facilitates the detection of low-abundance SARS-CoV-2 pseudoviruses. Ct Value 0 copies/ml 125copies/ml 250 copies/ml 500 copies/ml 1000 copies/ml NTC Carrier RNA(+) N/A; N/A; N/A N/A; N/A; N/A N/A; 40.4; N/A N/A; 40.16; 40.88 38.05;38.02 N/A; N/A; N/A Carrier RNA(-) N/A; N/A; N/A N/A; N/A; N/A 40.2; 40.29; 38.95 38.61; 39.03; 38.59 37.53;36.34 B. YeasenBio Carrier RNA V.S.Supplier Q Products The Magnetic Bead Swab Virus DNA/RNA Extraction Kit was used to extract different concentrations of nucleic acid from SARS-CoV-2 pseudovirus samples, and the Detection Kit was then used to detect the N gene. The results showed that after the addition of carrier RNA, the extraction efficiency was higher, making the downstream detection more sensitive. Ct Value 0 copies/ml 125copies/ml 250 copies/ml 500 copies/ml 1000 copies/ml NTC YeasenBio N/A; N/A; N/A N/A; 40.01; N/A 38.76; 38.47; 39.53 38.83; 37.60; 37.82 37.91;37.17 N/A; N/A; N/A Supplier Q N/A; N/A; N/A N/A; N/A; N/A N/A; 39.57; N/A 37.42; 39.06; 38.85 N/A;36.04
