Ceturegel™ Matrix GFR, Phenol Red-Free, LDEV-Free _ 40186ES

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Ceturegel™ Matrix GFR LDEV-Free Matrix is a growth factors reduced soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG) and nestin. Ceturegel™ GFR Matrix is a low-growth factor Matrix obtained after special treatment, which can effectively ensure the needs of some experimental operations that require low-growth factors, such as experiments related to signaling pathways and growth factors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for cell morphology, biochemical function, migration, Invasion and gene expression studies. The three-dimensional culture matrix formed by Matrix can promote the adherence and differentiation of epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells, and hair follicle cells. Ceturegel™ Matrix can affect gene expression in three-dimensional cultures of murine hepatocytes and human mammary epithelial cells. At the same time, Ceturegel™ Matrix can be used as a basic scaffold for various tumor cell invasion studies using matrigel, an essential matrix for in vitro and in vivo studies of angiogenesis, and a three-dimensional scaffold for the growth of transplanted tumor cells in in vivo animal models. Ceturegel™ Matrix also supports the regeneration of peripheral nerves and the differentiation of bovine fallopian tube epithelial cells. YEASEN Matrix is a sterile product, does not contain phenol red, and has a concentration of 8~12 mg/mL, which meets various experimental requirements. Feature High Safety: No LDEV (lactate dehydrogenase elevating virus) Multiple Concentration: the concentration range from 8-12 mg/mL Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc. Low Endotoxin: Endotoxin content 50L per single batch Wide Compatibility: Compatible with almost all types of cell culture medium Application 2D Culture Support Migration/Invasion Biochemical Research 3D & Organoid Culture Angiogenesis In Vivo Tumor Formation Specification Concentration 8-12 mg/mL Product Type Basement Membrane Matrix Endotoxin Level Low Transportation Conditions Dry Ice Transportation Product Line Ceturegel™ Form Frozen Phenol Red Indicator None Product specifications 5/10 mL Species EHS Mouse Tumors Classification Low Growth Factor Serum Level None LDEV Detection None Components Components No. Name 40186ES08 40186ES10 40186 Ceturegel™ Matrix GFR，Phenol Red-Free，LDEV-Free 5 mL 10 mL Figures 1. Angiogenesis Case Study 40186 is a low-growth factor formula that reduces interference from exogenous VEGF/FGF, features rapid lumen formation and clear networks, and is suitable for anti-angiogenic drug screening and endothelial cell function research. Figure 1. Angiogenesis Case Study Thaw the Matrigel overnight at 4°C. Coat each well of a 24-well plate with 100-150 μL of gel and incubate at 37°C for 30-60 minutes to solidify. Use passage 3-5 HUVECs at 70%-80% confluence, harvest and resuspend the cells, and seed them at a density of 1.2×10⁵ cells/well. Observe the cells every 4 hours post-seeding; tube formation is typically visible within 4-18 hours. 2. Angiogenesis(Cat#40186ES) HUVEC Angiogenesis Assay Cas 1 Cas 4 Cas 7 Cas 10 Cas 2 Cas 5 Cas 8 Cas 11 Cas 3 Cas 6 Cas 9 Show more