One-step rapid mycoplasma detection kit (LAMP) (2G) is a rapid test for mycoplasma contamination in cell culture based on the LAMP method. The kit uses Yeasen's unique loop-mediated isothermal amplification (LAMP) technology to design specific primers for the conservative region of the mycoplasma 16S-23S rRNA sequence. It can directly use biological materials such as cell culture supernatant or serum as templates. If it is contaminated with mycoplasma, the conservative sequence of mycoplasma DNA will be amplified in large quantities and rapidly, turning the reaction solution from blue-purple to sky blue. The results can be distinguished by the naked eye without the need for electrophoresis. This kit can detect 33 types of mycoplasma, including oral mycoplasma (M.orale), pneumoniae (M.pneumoniae), hyorhinis (M.hyorhinis), salivarium (M.salivarium), and arginini (M.arginini) required by domestic and foreign pharmacopoeia regulations. The sensitivity can reach 500 copies/uL. It can also amplify and react to 100 CFU/mL mycoplasma standard strains normally. It has the characteristics of strong specificity, high sensitivity, convenient and fast operation. Compared with one-step rapid mycoplasma detection kit(1G), this product has been optimized and upgraded to reduce the false positive rate, improve the accuracy of the test results, and enhance the color recognition of negative and positive. After the amplification, the negative will not turn into positive after being placed at room temperature for a period of time, which does not affect the judgment of negative and positive. Features Simple and rapid: One-step detection with easy-to-use protocol Broad compatibility: Directly applicable to culture supernatant, serum, and other sample types Extensive coverage: Capable of detecting 33 mycoplasma species High sensitivity: Detects as low as 500 copies/μL or 100 CFU/mL with a clear colorimetric change Safe & Contamination-Free: Contains UDG anti-contamination and non-infectious positive controls to ensure safety and eliminate the risk of cross-contamination Specifications For Use With (Equipment) Water bath or metal bath or PCR instrument Sample Type Strains, cell supernatants, serum etc. Polymerase Bst DNA Polymerase Detection Method LAMP Detection limit 500 copies/μL Detection of mycoplasma types 33 types, including 9 types of mycoplasma covered by the pharmacopoeia Reaction time (fast PCR procedure) 30 min Components Components No. Name 40615ES08 40615ES25 40615ES60 40615-A MycAway™ -Color LAMP Buffer 85 μL 425 μL 425 μL×4 40615-B MycAway™ -Color LAMP Enzyme 5 uL 25 μL 25 μL×4 40615-C MycAway™ -Color LAMP Primer 10 μL 50 μL 50 μL×4 40615-D Mineral oil 100 μL 500 μL 500 μL×4 40615-E Positive Control 10 μL 10 μL 10 μL×4 Application mycoplasma contamination; mycoplasma testing; Cell Culture Quality Control Figures 1. Product Performance Summary Detection Range Capable of detecting Mycoplasma pneumoniae, Mycoplasma orale, Mycoplasma hyorhinis, Mycoplasma salivarium, and Mycoplasma arginini Cross-Reactivity (Cross-reaction with other DNAs) No cross-reactivity with DNA from close relative strains, common engineered bacteria, or engineered cell lines Anti-Interference Capability (Interference of different sample matrices on mycoplasma detection) Detection is unaffected by matrices such as cell culture media Limit of Detection (LOD) Limit of detection down to E2 copies/μL Durability (Accelerated thermal stability, freeze-thaw stability, transportation stability) Product sensitivity and negative/positive controls meet the requirements Competitor Comparison Negative (bluish-purple), Positive (sky-blue); distinct sample color contrast, high detection sensitivity 2. Extensive coverage: Capable of detecting 33 mycoplasma species 3. Advanced detection capabilities Figure 1. The MycAway™ Plus-Color One-Step Mycoplasma Detection Kit (2G) provides robust detection performance than kits from other suppliers. Using the same 100 CFU standard sample, 40615 reagent detects 100 CFU standard, which is not detectable by Supplier B*. Compared to Supplier A*, 40615 shows a more distinct color change with the same sample. 4. Superior inhibitor tolerance Figure 2. Matrix tolerance of MycAway™ Plus-Color One-Step Mycoplasma Detection Kit (2G) 5. Stability Figure 3. Stability Test (A) Freeze-thaw stability: The kit withstands 20 freeze-thaw cycles at -25 to -15°C without loss of performance. (B) Heat-accelerated stability: The One-Step Mycoplasma Detection Kit (2G) remains stable after 14 days at 37°C.
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