Hieff Trans™ Liposomal Transfection Reagent is a versatile liposome transfection reagent, suitable for DNA, RNA, and oligonucleotide transfection, with high transfection efficiency for most eukaryotic cells. Its unique formula allows it to be added directly to the medium, and the presence of serum does not affect transfection efficiency, which reduces the damage to cells caused by serum removal. There is no need to remove the nucleic acid-Hieff Trans™ complex or replace it with a fresh medium after transfection, and it can also be removed after 4-6 hours. Hieff Trans™ is supplied in sterile liquid form. Usually, for 24-well plate transfection, about 1.5 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections; for a 6-well plate, about 6 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections. 160 transfections. Feature High Efficiency: Enables transfection in most eukaryotic cells, achieving over 90% efficiency in common cell lines. Low Toxicity: Transfected cells maintain healthy morphology while expressing abundant target protein. Easy to Use: Lipid complexes can be directly added to serum-containing culture media. Versatile Applications: Suitable for both transient and stable transfection experiments. Application Cell Transfection Specification Form Liquid Serum Compatible Yes Cell Type Established Cell Lines Sample Type Plasmid DNA, Synthetic siRNA Transfection Technique Lipid-Based Transfection Components Components No. Name 40802ES01 40802ES02 40802ES03 40802ES08 40802 Hieff Trans™ Liposomal Transfection Reagent 100 μL 0.5 mL 1 mL 5×1 mL Figures 1. High transfection efficiency Figure 1. Efficient DNA transfection in 293 cells. Transient transfection was performed in 12-well plates using a total of 1 μg plasmid (0.5 μg GFP + 0.5 μg RFP) with 3 μL of transfection reagent. Cited from: Communications Biology volume 5, Article number: 1296 (2022) 2. High transfection siRNA Transfection Figure 2. Efficient AMPK gene silencing in HK2 cells. HK2 cells were transfected in 6-well plates with 100 pmol siRNA targeting AMPK using 6 μL of transfection reagent. High silencing efficiency (+++) was observed with no detectable cytotoxicity Each reagent was used to transfect the target cell line（Hela cell line in Figure 1a, DF-1 cell line in Figure 1b）in a 96-well format. GFP expression was analyzed 48 hours posttransfection. Hieff Trans™ Liposome Transfection Reagent provided higher GFP transfection efficiency than the competitive brand products. 3. Figure 3. Comparison of transfection efficiency between Hieff Trans™ Liposome Reagent and other brands. (A).Hieff Trans™ and supplier P* were used to transfect HeLa and DF-1 cells in 96-well plates. GFP expression was measured 48 hours post-transfection. Hieff Trans™ consistently achieved higher GFP transfection efficiency than the other products. 4. Validated Cell Lines 293T 293FT HEK293 HEK 293T Caco2 COS-7 DF-1 Lenti X-293T HeLa HepG2 LM3 MCF-7 MDA-MB-231 MEF NCI-H1975 NIH-3T3 PC12 Raw264.7 Vero H9c2 Hepa1-6 MCF10A CHO 5-8F BV-2 A549 3t3 H520 HUVEC C6 HaCaT N2A SGC-7901 H9 MKN-28 Hep 3B SMCC7721 RKO C2C12 More.. Figure 4. List of cell lines successfully transfected with Hieff Trans™ Liposomal Transfection Reagent (Under continuous update). 5.Case
