PEI 40000 is a highly charged cationic polymer with a molecular weight of 40,000 that binds negatively charged nucleic acid molecules very easily, forming a complex and allowing the complex to enter cells. PEI 40000 is a transient transfection reagent with low cytotoxicity, high transfection efficiency, and high gene expression efficiency in cells such as HEK293 and CHO. PEI 40000 has many advantages over PEI 25000 Transfection Reagent. include: 1. PEI 40000 is easy to dissolve and can be directly dissolved in water. PEI 25000 needs to first adjust the water to weak acid to help it dissolve, and then use NaOH to adjust the pH to neutral. 2. PEI 40000 is easy to operate, easier to use, and has a better transfection effect than PEI 25000; 3. PEI 25000 contains 4-11% propionyl residues, which can prevent the polymer backbone from binding to DNA. Compared to PEI 25000, PEI 40000 is a complete shed construction, so its performance is consistently efficient. This product is an instant type, which dissolves quickly and is easy to prepare. Feature High Transfection Efficiency: Ideal for DNA transfection; transfection efficiency can reach over 80% when cell density is at 70%–80%. Broad Applications: Suitable for both adherent and suspension cells, and compatible with both transient and stable transfection. Low Cytotoxicity: Transfected cells maintain good morphology and express high levels of target proteins. Easy Protocol: Solutions can be prepared directly with distilled water, and complexes can be added directly to serum-containing media. Cost-Effective: At the same volume/specifications, the price is one-third of the competitor's poly product, making it ideal for large-scale transfection. Application Cell Transfection Specification Name Polyethylenimine Linear (PEI) 40000 CAS No. 49533-93-7 Molecular formula (CH₂CH₂NH)n Molecular weight 40,000 Solubility Soluble in water, insoluble in organic solvents: benzene, ether and acetone Structure Form White or off-white solid Serum Compatible Yes Cell Type Established Cell Lines Sample Type Plasmid DNA Transfection Technique PEI-Based Transfection Components Name 40816ES01 40816ES02 40816ES03 40816ES10 Polyethylenimine Linear(PEI) MW40000 (Rapid Dissolving) 5 mg 100 mg 1 g 10 g Figures 1. Demonstration of Transfection Effect Figure 1. The transfection efficiency of Polyethylenimine Linear (PEI) MW40000 Reagent was shown above. Polyethylenimine Linear (PEI) MW40000 Reagents were used to transfect the HEK293 cell line and CHO-K1 cell line in a 96-well format. GFP expression was analyzed 48 hours posttransfection. 2. Validated Cell Lines Product Name Polyethylenimine Linear (PEI) MW40000 (Rapid Lysis) Validated Cell Lines COS-1 CHO-K1 HEK293 HepG2 COS-7 Hela HEK293T NIH-3T3
Related
Related
Related