This product is specifically developed for the large-scale production of lentiviral vectors (LV). It is an optimized, linearized polyethylenimine (Linear PEI). Synthesized entirely through chemical processes, this product is free of animal-derived components and exhibits low cytotoxicity. It is provided as a sterile liquid solution and is fully compatible with both suspension and adherent HEK293 cell systems for LV packaging. Featuring high DNA loading capacity, a low transfection complex ratio, and high complex stability, it greatly facilitates large-scale LV production. Features Higher Viral Yield: Increases viral production by 2-3 fold. Broader Applicability: Suitable for lentiviral (LV) vector preparation in both adherent and suspension cell systems. Superior Compatibility: Delivers enhanced viral yields for both 4-plasmid and 5-plasmid packaging systems. Specifications Cat.No. 40825ES01 / 40825ES03 / 40825ES10 / 40825ES60 Size 100 μL / 1 mL / 10 mL / 100 mL Components Components No. Name 40825ES01 40825ESO3 40825ES10 40825ES60 40825 Hieff Trans™ UltraLV Transfection Reagent 100 μL 1 mL 10 mL 100 mL Figures HEK293 (G2S) case study Figure. Enhanced Lentiviral (LV) Yield Under identical experimental conditions (with only the transfection reagent varied), Yeasen reagent achieves a 2-fold increase in lentiviral titer compared to leading commercial alternatives. Figure. Simplified One-Step Transfection Workflow. The one-step protocol streamlines the process by directly adding the transfection reagent and plasmids into serum-free medium for co-incubation. This eliminates the separate dilution steps required in traditional two-step methods, significantly reducing pipetting and saving time. Figure. High Stability of Transfection Complexes. The transfection complexes exhibit excellent stability, with viral yields remaining consistent whether incubated for 15 or 30 minutes. This demonstrates a broad operational window, providing greater procedural flexibility and accommodating varying laboratory workflows. Figure. Minimal Cytotoxicity at High Reagent Ratios. Increasing the transfection reagent volume does not compromise cell health. At 48 hours post-transfection, cell viability remains stable at both 1:2 and 1:3 plasmid-to-reagent ratios, confirming the reagent's exceptionally low toxicity. Figure Consistent performance across different batches
