E.coli Host Cell RNA Residue Detection Kit _ 41318ES

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E.coli Host Cell RNA Residue Detection Kit is used for the quantitative analysis of E.coli host cell RNA residuce in intermediate samples, semi-finished and finished products of va rious biological products. This kit adopts Taqman fluorescent probe and the polymerase chain reaction (PCR) method, which has fg level minimum detection limit and can specifically and quickly detect the residual E.coli cell RNA. The kit needs to be used together with the the Residual DNA Sample Preparation Kit (Cat# 18461ES) and Recombinant DNase I (RNase-free, Yeast) (Cat#14534ES50/14534ES60). Feature Compliance with regulations: the products are fully validated according to the requirements of Chp, USP, ICH, etc., and their performance is in line with Chinese and foreign regulations and standards; Guarantee of quality: the raw materials of the kits are all self-developed, and the qPCR Mix and other enzyme products are produced in an ultra-clean enzyme factory; High sensitivity: the limit of quantification can reach 1 fg/μL level; High precision: high intra-batch repeatability and low inter-batch variation; Strong exclusivity: specific detection of residual RNA in E.coli cells without interference from other exogenous genomic DNA and RNA; Strong anti-interference: add internal control (IC), easy to exclude sample interference, reaction preparation abnormalities and other factors. Application Residual E.coli Host Cell RNA test in biological products. Specification Sensitivity 1 fg/μL (range 2fg/μL~200pg/μL) Assay Time ~1.5 hours Assay Principle Fluorescent probe qPCR method Required Instruments Real-time fluorescence quantitative PCR system Components Components No. Name 41318ES50-EN 41318ES60-EN 41318-A E.coli qPCR Mix 0.75 mL 1.5 mL 41318-B One Step Enzyme Mix 200 μL 400 μL 41318-C RNA Dilution Buffer 1.8 mL×2 1.8 mL×4 41318-D E.coli RNA Control（20 ng/μL） 25 μL 50 μL 41318-E IC* 50 μL 100 μL *IC：Internal control. Figures Limit of quantification E.coli RNA was detected at 5 fg/uL, 2 fg/uL, and 1 fg/uL, with 10 replicates for each concentration. The results showed that the CV was Show more