In vitro cell culture often involves the addition of antibiotics to the culture medium to prevent microbial contamination, with penicillin being the most widely used. The mechanisms of action of penicillin and streptomycin involve inhibiting bacterial protein synthesis, while amphotericin B can inhibit the growth of fungi. This product has been sterilized by filtration through a 0.1 μm filter and contains Penicillin at 10,000 IU/mL, Streptomycin Sulfate at 10 mg/mL, and Amphotericin B at 25 μg/mL. It is provided as a 100X concentrated solution, which requires dilution before use, and it should be used without repeated freeze-thaw cycles. Yeasen company provides three commonly used multi-antibiotic solutions for cell culture: 60162ES, 60290ES and 60291ES. The differences among these three products are as follows: Product Number 60162ES 60290ES 60291ES Product Name Penicillin-Streptomycin Solution, 100×, Suitable for Cell Culture Penicillin-Streptomycin-Neomycin Solution(PSN), 100×, Suitable for Cell Culture Penicillin-Streptomycin-Amphotericin B Solution, 100×, Suitable for Cell Culture Main Active Ingredients Penicillin 10,000 U/mL; Streptomycin 10,000 µg/mL Penicillin G Sodium Salt 5 kU/mL; Sulfate Streptomycin 5 mg/mL; Sulfate Neomycin 10 mg/mL Penicillin 10,000 IU/mL; Sulfate Streptomycin 10 mg/mL; Amphotericin B 25 µg/mL Solvent Sterile Water PBS Buffer Solution Cell Culture Medium Water Recommended Usage Concentration Dilute to 1× Applicable Scenarios Effectively inhibits the growth of most Gram-positive and Gram-negative bacteria Have a good combined antibacterial effect against Gram-positive and Gram-negative bacteria Penicillin and streptomycin inhibit bacterial protein synthesis, and amphotericin B inhibits the growth of fungi For other antibiotics, please refer to the related topic of Antibiotic User Manual. Features Standardized production Penicillin 10,000 IU/mL; Sulfate Streptomycin 10 mg/mL; Amphotericin B 25 µg/mL Applications Cell culture Penicillin and streptomycin inhibit bacterial protein synthesis, and amphotericin B inhibits the growth of fungi Specifications Size 100 mL Appearance Liquid pH 5.2-7.0 Components Components No. Name 60291ES60 60291 Penicillin-Streptomycin-Amphotericin B Solution, 100×, Suitable for Cell Culture 100 mL Shipping and Storage The Penicillin-Streptomycin-Amphotericin B Solution products be stored at -25～-15℃ for 3 years, protected from light. Documents: Safety Data Sheet 60291_MSDS_HB250210_EN.pdf Manuals 60291_Manual_HB250210_EN.pdf
