Interleukin-4 (IL-4) is a cytokine that belongs to the interleukin family. Interleukin 4 is mainly produced by T cells, B cells, macrophages and other immune cells.IL4 is a key regulatory molecule that plays an important role in the Th2 type of immune response. Interleukin 4 has a variety of biological functions that promote B cell class switching to IgE and IgG1, thereby enhancing the effects of humoral immunity. In addition, IL4 inhibits the activation of macrophages and dendritic cells and suppresses their release of inflammatory cytokines. In clinical practice, interleukin 4 is widely used in the treatment of allergic diseases, such as asthma and allergic rhinitis. This product, Recombinant Human 4, is supplied as a lyophilized powder with high activity, high purity and low endotoxin. Specification Synonyms Interleukin-4, IL-4, BSF-1, BCDF, IL4E12, Pitrakinra Source E.coli-derived human 4 protein, His25-Ser153. Tag Not Accession P05112 Molecular Weight Approximately 15.0 kDa. AA Sequence HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE NFLERLKT IM REKYSKCSS Endotoxin < 1.0 EU/μg by LAL method Purity > 98% by SDS-PAGE and HPLC. Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Physical Appearance White lyophilized powder Storage -25 ~ -15 ° C storage, valid for 1 year after receipt. After re-solution, store at 2 ~8 °C, 7 days expiration date. After re-dissolution, store at -85~-65°C, 3 months expiration date. Compounding Methods Centrifuge before uncapping to bring contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL, which can be diluted even further according to subsequent experiments; for long-term storage, it is recommended that carrier proteins (0.1% BSA) be added to the dilution buffer; dispense for a single experimental dose and freeze at -80°C to avoid repeated freezing and thawing. Cautions 1. Please operate with lab coats and disposable gloves,for your safety. 2. This product is for research use only.
