Interleukin-5 (IL-5) is a hematopoietic growth factor that stimulates the proliferation and activation of eosinophils.IL-5 is produced by mast cells, T-cells, and eosinophils, and plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors.Elevated levels of IL-5 can lead to eosinophilia, which may cause asthma and other allergic diseases. Human and mouse IL-5 are cross-species reactive. Recombinant Human IL-5 is a 26.5 kDa disulfide-linked homodimeric protein containing two 116 amino acid chains. This product, Recombinant Human IL-5, is supplied as a lyophilized powder with high activity, high purity and low endotoxin. Specification Synonyms Interleukin-5; B-cell differentiation factor I, Eosinophil differentiation factor, TRF Source E.coli-derived human IL-5 protein, Ile20-Ser134,with an N-terminal Met. Accession P05113 Molecular Weight Approximately 26.5 kDa. AA Sequence MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES Endotoxin < 1.0 EU/μg by the LAL method. Purity > 98% by SDS-PAGE and HPLC. Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.5, 3% trehalose, 0.1% Tween-80. Physical Appearance White lyophilized powder Storage -25 ~ -15 ° C storage, valid for 1 year after receipt. After re-solution, store at 2 ~8 °C, 7 days expiration date. After re-dissolution, store at -85~-65°C, 3 months expiration date. Compounding Methods Centrifuge before opening the cap to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL, which can be diluted even further according to subsequent experiments; for long-term storage, it is recommended that carrier protein (0.1% BSA) be added to the dilution buffer; dispense for a single experimental dose and freeze at -80°C to avoid repeated freezing and thawing. Cautions 1. Please operate with lab coats and disposable gloves,for your safety. 2. This product is for research use only.
