Interleukin-1β (IL-1β) is a pro-inflammatory cytokine produced by a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. IL-1α and IL-1β bind to the same receptor and exhibit similar biological properties, although they are not entirely identical. These cytokines display a broad spectrum of activities, including stimulating thymocyte proliferation by inducing IL-2 release, promoting B cell maturation and proliferation, exhibiting mitogenic FGF-like activity, and inducing the release of prostaglandins and collagenases from synovial cells. This recombinant human IL-1β is supplied as a lyophilized powder. It features high activity, high purity, low endotoxin levels, and is tag-free. Components Components No. Name 90140ES05 90140ES10 90140ES50 90140ES60 90140ES76 90140 Recombinant Mouse IL-1β Protein 2 μg 10 μg 50 μg 100 μg 500 μg Specifications Synonyms Interleukin-1 beta, IL-1β, IL-1, IL1B, IL-1b, IL1-BETA, IL-1F2, IL1F2IL-1 beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta. Source E.coli-derived Mouse IL-1β protein, Vla118-Ser269. Accession P10749 Molecular Weight Approximately 17.5 kDa. AA Sequence VPIRQLHYRL RDEQQKSLVL SDPYELKALH LNGQNINQQV IFSMSFVQGE PSNDKIPVAL GLKGKNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKSKV EFESAEFPNW YISTSQAEHK PVFLGNNSGQ DIIDFTMESV SS Endotoxin < 1.0 EU/μg by the LAL method. Purity > 96% as determined by SDS-PAGE and HPLC analyses. Biological Activity The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/mL, corresponding to a specific activity of > 5.0 × 108 IU/mg. Fully biologically active when compared to standard. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5% trehalose, 0.02% Tween-20. Tag No Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Storage This product should be stored at -25~-15℃ for 1 year. Once reconstituted under sterile conditions, it can be stored at 2 ~ 8°C for 7 days, or at -85 to -65°C for 3 months. Reconstitution Instructions Centrifuge the vial briefly before opening to pellet the contents at the bottom. Reconstitute with sterile deionized water to a final concentration of 0.1–1.0 mg/mL. Further dilution can be performed as needed for downstream applications. For long-term storage, it is recommended to add a carrier protein (e.g., 0.1% BSA) to the dilution buffer. Aliquot the solution into single-use portions and store at -80°C to avoid repeated freeze-thaw cycles. Notes 1. This product is for research use only. 2. Please operate with lab coats and disposable gloves，for your safety. Documents: Safety Data Sheet 90140_MSDS_HB260622 Manuals: 90140_Manual_HB20260622
