Interleukin-2 (IL-2) is a potent immunomodulatory lymphocyte cytokine produced by T cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is essential for T cell proliferation and other immune responses. The IL-2 receptor is composed of three subunits (55 kDa IL-2Rα, 75 kDa IL-2Rβ, and the 64 kDa common gamma chain γc/IL-2Rγ), which exist on the cell surface as distinct preformed complexes. This recombinant mouse IL-2 contains 149 amino acid residues and is supplied as a lyophilized powder. It features high activity, high purity, and low endotoxin levels. Components Components No. Name 90142ES08 90142ES10 90142ES50 90142ES60 90142ES76 90142 Recombinant Mouse IL-2 Protein 2 μg 10 μg 50 μg 100 μg 500 μg Specifications Synonyms Interleukin-2,IL-2,T-cell Growth Factor, TCGF, Aldesleukin; Expression Region & Expression System Mouse IL-2 Protein is expressed from E.coli with no tag .It contains Ala21-Gln169. Accession P04351 Molecular Weight Approximately 17.2 kDa AA Sequence APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF IS NIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ Endotoxin < 1.0 EU/μg by the LAL method. Purity > 95%(determined by SDS-PAGE and HPLC) Biological Activity The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg. Fully biologically active when compared to standard. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Physical Appearance White lyophilized powder Storage This product should be stored at -25~-15℃ for 1 year. Once reconstituted under sterile conditions, it can be stored at 2 ~ 8°C for 7 days, or at -85 to -65°C for 3 months. Reconstitution Instructions Centrifuge the vial briefly before opening to pellet the contents at the bottom. Reconstitute with sterile deionized water to a final concentration of 0.1–1.0 mg/mL. Further dilution can be performed as needed for downstream applications. For long-term storage, it is recommended to add a carrier protein (e.g., 0.1% BSA) to the dilution buffer. Aliquot the solution into single-use portions and store at -80°C to avoid repeated freeze-thaw cycles. Notes 1. This product is for research use only. 2. Please operate with lab coats and disposable gloves，for your safety. Documents: Safety Data Sheet 90142_MSDS_HB260622 Manuals: 90142_Manual_HB20260622
