Interleukin-3 (IL-3) is a hematopoietic growth factor that promotes the survival, differentiation, and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil, and mast cell lineages. IL-3 is produced by T cells, mast cells, and eosinophils, and can enhance thrombopoiesis, phagocytosis, and antibody-mediated cytotoxicity. Its ability to activate mononuclear cells suggests that IL-3 may have additional immunomodulatory roles. Many IL-3 activities depend on co-stimulation with other cytokines. Recombinant IL-3 is a 15.0 kDa recombinant protein containing 133 amino acid residues. This product is expressed in E. coli and provided as a lyophilized powder. It features high activity, high purity, low endotoxin, and is tag-free. Specifications Cat.No. 90212ES10 / 90212ES50 / 90212ES60 / 90212ES80 Size 10 μg / 50 μg / 100μg / 1 mg (10×100 μg) Components Name 90212ES10 90212ES50 90212ES60 90212ES80 Recombinant Human IL-3 GMP Protein 10 μg 50 μg 100 μg 1 mg (10×100 μg) Performance parameters Synonyms Interleukin-3; IL-3; Hematopoietic growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor Source E. coli Sequence Ala20-Phe152. Molecular Weight Approximately 15.08 kDa. Accession P08700 Tag No Tag Biological Activity The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7×106 IU/mg Purity > 95% as determined by SDS-PAGE. Endotoxin < 0.01 EU/μg protein as determined by LAL method. Sterility Negative. Mycoplasma Negative. In Vitro Virus Negative. Host Cell Protein Less than 0.5 ng/ug when tested by ELISA. Host Cell DNA Less than 20 ng/mg when tested by qPCR. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0. Physical Appearance Sterile filtered white lyophilized (freeze-dried) powder. Reconstitution Method It is recommended to reconstitute Human IL-3 in sterile PBS to a concentration of 0.1-1.0 mg/mL. Avoid repeated freeze-thaw cycles as much as possible. Product Data SDS- PAGE Human IL-3 on SDS-PAGE under reduced condition. The purity is greater than 95%. Cell Based Assay The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7 × 106 IU/mg. Notes 1. This product is for research use only. 2. Please operate with lab coats and disposable gloves,for your safety.
