Interleukin-6 (IL-6) is a pleiotropic cytokine that plays an important role in host defense by modulating immune and inflammatory responses.IL-6 is produced by T cells, monocytes, fibroblasts, endothelial cells, and keratin-forming cells, and has a variety of biological functions. It stimulates B cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces the expression of hepatic acute-phase proteins, and regulates bone metabolism.IL-6 signals through the IL-6 receptor system, which consists of two chains, IL-6Rα and gp130. This product, Recombinant Human IL-6, is supplied as Lyophilized powder with high activity, high purity and low endotoxin. Product Properties Synonyms Interleukin-6,BSF2,HSF,IFNB2;IL6 Species Human Source CHO Cells Sequence Val30-Met212. Accession P05231 Tag NO Molecular Weight Predicted molecularmass：20.8 kDa Endotoxin ≤10 EU/mg by the LAL method Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) Activity Measured in a cell proliferation assay using M-NSF-60 cells. The ED50 forthis effect is 0.2-1 ng/mL Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitoland 0.01% Tween 80, pH 7.4 Appearance Lyophilized powder Shipping and Storage -25 ~ -15 ° C storage, from the date of receipt of goods, the expiration date of 1 year. After re-solution, store at 2 ~8 °C, 7-10 days expiration date. After re-dissolution, -85 ~ -65 ° C storage, 6 months expiration date. Compounding method Use NO bacterial deionized water to reconstitute lyophilized proteins. It is recommended that proteins be divided for first use to avoid repeated freeze-thawing. Figure Figure 1.Measured in a cell proliferation assay using M-NSF-60 cells. The ED50 forthis effect is 0.2-1 ng/mL. Figure 2.Human IL-6 Protein on SDS-PAGE under reducing (R) condition. The purity is greater than 95%. Cautions 1. For your safety and health, please wear lab coat and disposable gloves. 2. This product is for scientific research purposes only.
