Recombinant Mouse IP-10/CXCL10 Protein _ 90927ES

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CXCL10 is a cytokine belonging to the CXC chemokine family. Mouse CXCL10 shares approximately 67% amino acid sequence identity with human CXCL10. CXCL10 has been shown to be a chem oattractant for activated T-lymphocytes and monocytes. It also has the effect of promoting the adhesion of T cells to endothelial cells, as well as inhibiting bone marrow colony formation and angiogenesis. Product Properties Synonyms Gamma-IP10, Small-inducible Cytokine B10, C7, CXCL10, gIP-10, IFI10, Interferon-gamma Induced Protein CRG-2 Accession P17515 GeneID 15945 Source E.coli-derived mouse IP-10/CXCL10 protein, Ile22-Pro98. Molecular Weight Approximately 8.7 kDa. AA Sequence IPLARTVRCN CIHIDDGPVR MRAIGKLEII PASLSCPRVE IIATMKKNDE QRCLNPESKT IKNLMKAFSQ KRSKRAP Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >97% by SDS-PAGE and HPLC analyses. Biological Activity The biologically active determined by a chemotaxis bioassay using human peripheral blood lymphocytes is in a concentration range of 0.1-10.0 ng/mL in the presence of IL-2. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ for 1 year. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1.For your safety and health, please wear lab coats and disposable gloves for operation. 2.Forresearch use only! Show more