Interferon-γ(IFN-γ) is an immunomodulatory protein that belongs to the interferon family. Interferon-γ is mainly produced by activated T cells, NK cells and certain subpopulations of lymphocytes, and is a cytokine with multiple biological functions. Interferon gamma plays an important regulatory role in the immune response. It enhances the killing capacity of macrophages and dendritic cells, promotes their release of inflammatory cytokines, and regulates the expression of antigen-presenting molecules, thereby strengthening the cellular immune response. In addition, interferon gamma can regulate the differentiation and function of T cells, promote the development of Th1 cells, enhance B cell class switching to IgG, and regulate the balance between cellular and humoral immunity. In clinical practice, interferon γ is widely used in the treatment of certain infectious diseases and malignant tumors, especially those related to cellular immunity. This recombinant human IFN-γ is supplied in the form of lyophilized powder with high activity, high purity, low endotoxin and no redundant labeling. Specification Synonyms Interferon-gamma, IFN-γ, IFG, IFI, IFNG, IFNgamma, IFN-gamma. Source E.coli-derived human IFN-γ protein, Gln24-Gln166 with an N-terminal Met. Accession P01579 Molecular Weight Approximately 16.9 kDa. AA Sequence MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKNFKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ Endotoxin < 1.0 EU/μg by the LAL method. Purity > 98% by SDS-PAGE and HPLC Biological Activity The ED50 as measured in anti-viral assays using human HeLa cells infected with encephalomyocarditis (EMC) virus is 0.15-0.80 ng/ml. Fully biologically active when compared to standard. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Storage -25 ~ -15 ° C storage, 1 year expiration date after receipt. After re-solution, store at 2 ~8 °C, 7 days expiration date. After re-dissolving, store at -85~-65°C, 3 months expiration date. Compounding Methods Centrifuge before uncapping to bring contents to the bottom. Use sterile deionized water to reconstitute and reconstitute to a concentration of 0.1-1.0 mg/mL, which can be diluted even further according to subsequent experiments; for long-term storage, it is recommended that carrier protein (0.1% BSA) be added to the dilution buffer; dispense for a single experimental dose and freeze at -80°C to avoid repeated freezing and thawing. Cautions 1. Please operate with lab coats and disposable gloves,for your safety. 2. This product is for research use only.
