Thrombopoietin (TPO), is a key regulator of megakaryocytopoiesis and thrombopoiesis. The 353 amino acid (aa) human TPO precursor is cleaved to yield the 332 aa mature protein. Mature human TPO shares approximately 70% aa sequence homology with mouse and rat TPO. TPO and its receptor (c-Mpl) are the major regulators of megakaryocyte and platelet production and serve a critical and non-redundant role in hematopoietic stem cell (HSC) biology. TPO signals through the Jak-STAT, Ras-Raf-MAPK, and PI3K pathways, and promotes survival, proliferation, and polyploidization in megakaryocytes. The proto-oncogene c- also plays an important role in many of these same processes. Product Properties Synonyms MGDF, MGDFC-mpl ligand, MKCSF, MK-CSF, ML, MPL ligand, MPLLG, THCYT1, THPO Accession P40226 GeneID 21832 Source HEK293-derived mouse TPO protein, Met1-Thr365. Molecular Weight Approximately 85.0 kDa. AA Sequence MELTDLLLA AMLLAVARL TLSSPVAPA CDPRLLNKL LRDSHLLHS RLSQCPDVD PLSIPVLLP AVDFSLGEW KTQTEQSKAQDILGAVSL LLEGVMAAR GQLEPSCLS SLLGQLSGQ VRLLLGALQ GLLGTQLPL QGRTTAHKD PNALFLSLQ QLLRGKVRF LLLVEGPTL CVRRTLPTT AVPSSTSQL LTLNKFPNR TSGLLETNF SVTARTAGP GLLSRLQGF RVKITPGQL NQTSRSPVQ ISGYLNRTHG PVNGTHGLFA GTSLQTLE ASDISPGAF NKGSLAFNLQGGLPPSPS LAPDGHTPF PPSPALPTT HGSPPQLHPLFPDPSTTM PNSTAPHPV TMYPHPRNL SQET Tag C-His Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 90% by SDS-PAGE analyses. Biological Activity Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 1.6-6.4 ng/mL. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only!
