Leptin is a protein product of the mouse obese gene . It modulates multiple functions including energy homeostasis, thermoregulation, bone metabolism, endocrine, and pro-inflammatory immune responses. As a signal molecule with growth factor characteristics, leptin can stimulate osteoblastic cells and inhibit osteoclast formation and activity, thus promoting osteogenesis. Product Properties Synonyms Obese Protein, Obesity Factor Accession P41159 GeneID 3952 Source E.coli-derived Human Leptin, Val22-Cys167. Molecular Weight Approximately 16.0 kDa. Tag None Purity > 95% by SDS-PAGE. Biological Activity Immobilized Human Leptin Antibody at 3 μg/mL (100 μL/well) can bind Human Leptin , the EC50 of Human Leptin is 174-246 pg/mL. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 1%BSA. Reconstitution 1.We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS, pH 7.4, with 0.1% BSA to a concentration of 0.1-1.0 mg/mL. 2.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. 3.Further dilutions should be made in PBS, which must contain carrier proteins, such as 0.1% BSA, 10% FBS, 5% HSA, 5% trehalose, one of four options. 5% trehalose is recommended for experimental culture without serum. Shipping and Storage The products are shipped with dry ice and can be stored at -25~-15℃ for 1 year. 1 month, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1.For your safety and health, please wear lab coats and disposable gloves for operation. 2.For research use only! Assay Data HB23020
