This kit contains Recombinant Human IL-2, Human IL-12, and Human IL-15. It is designed for the isolation, activation, and expansion of human Natural Killer (NK) cells from healthy human peripheral blood mononuclear cells (PBMCs). IL-2: A T-cell growth factor that also effectively promotes NK cell proliferation and activation. It enhances NK cell cytotoxicity (perforin and granzyme expression); however, prolonged exposure to high concentrations of IL-2 may lead to activation-induced cell death and the expansion of Treg cells. IL-15: The natural survival and homeostatic factor for NK cells. In in vitro culture, it plays a key role in maintaining basal NK cell survival, preventing apoptosis, and sustaining proliferation. IL-12: A potent pro-inflammatory cytokine. It strongly induces IFN-γ production in NK cells and promotes the formation of "memory-like" or "adaptive" NK cells. Components Components No. Name 92652ES10 92652ES50 92652ES60 92652ES-A Human IL-2 Protein 10 μg 50 μg 100 μg 92652ES-B Human IL-15 Protein 10 μg 50 μg 100 μg 92652ES-C Human IL-12 Protein 10 μg 50 μg 100 μg Storage Storage: Store at -25°C to -15°C. Valid for 1 year from the date of receipt. After Reconstitution: Store at -85°C to -65°C under sterile conditions. Valid for 3 months. Product Information Original Cat. No. Product Name Source Appearance 90213ES Human IL-2 Protein E.coli Lyophilized Powder 90113ES Human IL-15 Protein E.coli Lyophilized Powder 90262ES Human IL-12 Protein CHO Cells Lyophilized Powder Notes 1. This product is for research use only. 2. Please operate with lab coats and disposable gloves，for your safety. Instructions Component Name Reconstitution Method Human IL-2 Protein Centrifuge the vial prior to opening to collect contents at the bottom. Reconstitute with sterile deionized water to a concentration of 0.1-1.0 mg/mL. For subsequent experiments, further dilutions can be made. For long-term storage, it is recommended to add a carrier protein (0.1% BSA) to the dilution buffer. Aliquot into single-use volumes, store at -80°C, and avoid repeated freeze-thaw cycles. Human IL-15 Protein Human IL-12 Protein Documents: Manuals 92652_Manual_HB20260403
