Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM-8), also known as CGM6 and CD66b, is an approximately 95 kDa member of the human carcinoembryonic antigen (CEA) family that plays a role in cell adhesion, cell migration and pathogen binding. CEACAM-8 is anchored to cells by a glycophosphatidylinositol (GPI) motif and is expressed exclusively on granulocytes. Human CEACAM-8 is composed of a 34 amino acid (aa) signal peptide, a 29 aa propeptide segment which is removed in the mature form, and a 286 aa CEACAM-8 region containing one V-type and two C2-type Ig-like domains. A homologue for CEACAM-8 has not been identified in the mice or rats. Activation enhances surface expression of CEACAM-8 and is an activation marker in human neutrophils and eosinophils. Engagement of CEACAM-8 by mAb or galectin-3 activated a Src family kinase (lyn), and induced cellular adhesion, superoxide production, and degranulation of eosinophils. CEACAM-8 can form both homotypic, and heterotypic bonds with CEACAM-6 through interaction of the V-type Ig-like domains. Cross-linking of neutrophil CEACAM-8 augments cytokine secretion and beta2 integrin-mediated adhesion. Specifications Synonyms CD66b; CEACAM8; CGM6; CD67; NCA-95 Uniprot No. P31997.2 Source Recombinant Human CEACAM-8/CD66b Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Gln35-Ser319. Molecular Weight Approximately 32.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. 外观 Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data：Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Human CEACAM-6, His Tag with an affinity constant of 4.59 μM as determined in SPR assay (Biacore T200). Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -20~-80℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3-6 months, -20~-80℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Avoid repeated freezing and thawing. 2.Please operate with lab coats and disposable gloves, for your safety. 3.This product is for research use only. Product Data
