The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR, HER2 , HER3 and HER4 . Within the ECD, human EGFR shares 88% aa sequence identity with mouse and rat EGFR. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. Specifications Synonyms ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2 Uniprot No. P00533-1 Source Recombinant Biotinylated Human EGFR/HER1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu25-Ser645. Source The protein has a predicted MW of 71.5 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC Activity SPR data: Biotinylated Human EGFR, His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Human EGF, No Tag with an affinity constant of 0.64 nM as determined in SPR assay. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage The products are shipped with ice pack and can be stored at -20℃ for 1 year. 2-7 days, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. Note 1.For your safety and health, please wear lab coats and disposable gloves for operation. 2.For research use only! Product Data
