Biotinylated Human HGFR/c-MET Protein, His-Avi Tag

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HGFR is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. The protein possesses tyrosine kinase activity. The pr imary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Within the ECD, human HGFR shares 86%-88% aa sequence identity with canine, mouse, and rat HGFR. Specifications Synonyms MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor Uniprot No. P08581-1 Source Recombinant Biotinylated Human HGF R/c-MET Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Glu25-Thr932. Molecular Weight The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 72.1 kDa (β chain). Due to glycosylation, the protein migrates to 45-50 kDa and 80-90 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC Activity ELISA Data: Immobilized Anti-HGF R Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human HGFR, His Tag with the EC50 of 25.7ng/ml determined by ELISA. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage The products are shipped with ice pack and can be stored at -20℃ for 1 year. 2-7 days, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only. Product Data Show more