Biotinylated Human Nectin-4 Protein, His-Avi Tag

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Nectin-4 (poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Human Nectin-4 mRNA is normally expre ssed in the placenta, especially in endothelial cells, while in the mouse it is found in the embryo, lung, testis and brain. In many human ductal breast or non-small cell lung carcinomas, Nectin4 is upregulated and a soluble 43 kDa form is found in the plasma. The extracellular domain of human Nectin-4 shares 91%, 92%, 93%, 91% and 90% amino acid sequence homology with the corresponding regions of mouse, rat, canine, porcine and bovine Nectin-4, respectively. In forming adherens junctions, trans interactions of Nectin-4 initiate cell-cell interactions and recruit intracellular cadherins through afadin and other junctional proteins. Specifications Synonyms EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4 Uniprot No. Q96NY8 Source Recombinant Biotinylated Human Nectin-4 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gly32-Val351. Source The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100μg/mL is recommended. Dissolve the lyophilized protein in distilled water. Storage The products are shipped with ice pack and can be stored at -20℃ for 1 year. 2-7 days, 2 to 8 °C under sterile conditions after reconstitution.3 -6 months, -20 to -80°C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. Note 1.For your safety and health, please wear lab coats and disposable gloves for operation. 2.For research use only! Product Data Show more