Specifications Synonyms Fibroblast growth factor receptor 2;FGFR-2;KGFR;CD332 Source Human FGFR2 beta (IIIb) Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Arg152-Glu378.[Accession | P21802-3] Molecular Weight The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on SDS-PAGE result. Endotoxin < 1EU per μg by the LAL method. Activity Blocking Data: Serial dilutions of Anti-FGFR2 Antibody were added into Human FGFR2 beta (lllb) , His Tag : Biotinylated Human FGF10, No Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 1.74ng/ml. ELISA Data: Immobilized Human FGFR2 beta (IIIb) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR2 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. SPR Data: Anti-FGFR2 Antibody, hFc Tag can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 1.98nM as determined in a SPR assay . Purity > 95% as determined by SDS-PAGE and by HPLC. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3-6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1. Please operate with lab coats and disposable gloves for your safety. 2. This product is for research use only.
