Trop-2,also known as epithelial glycoprotein-1 antigen (EGP-1),is a protein that in humans is encoded by the TACSTD2 gene.Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. Specifications Synonyms EGP1; EGP-1; TROP2; GA733-1; gp50; T16; TACSTD2; TROP-2; M1S1; TACD2 Uniprot No. P09758 Source Recombinant Biotinylated Human TROP-2/TACSTD2 Protein is expressed from HEK293 cells with His tag and Avi tag at the C-terminal. It contains His27-Thr274. Molecular Weight The protein has a predicted MW of 30.5 kDa. Due to glycosylation, the protein migrates to 46-55 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC Activity ELISA Data:Immobilized Anti-TROP-2 Antibody, hFc Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human TROP-2, His Tag with the EC50 of 24.1 ng/mL determined by ELISA. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage Transported with ice packs. Store at -20℃, valid for one year.。 After reconstitution, store unopened at -20 to -80°C for 3-6 months. After reconstitution, store at 2-8°C for 2-7 days. It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing. Note 1. Avoid repeated freezing and thawing. 2. For your safety and health, please wear a lab coat and disposable gloves when operating. 3. This product is for scientific research purposes only. Product Data
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