B-cell activating factor (BAFF) is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation (1-4). BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes. Specifications Synonyms CD268; BAFFR; BR3; TNFRSF13C; BROMIX; CVID4; prolixin Uniprot No. Q96RJ3-1 Source Recombinant Human BAFFR/TNFRSF13C Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Ser7-Ala71. Molecular Weight The protein has a predicted MW of 7.6 kDa. Due to glycosylation, the protein migrates to 13-25 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE Activity 1.ELISA Data: Immobilized Human BAFFR, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human BAFFR Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. 2.ELISA Data: Immobilized Human BAFF (Trimer) , hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 2.3μg/ml determined by ELISA. 3.SPR Data: Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF Trimer, hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage Transport with ice packs. Store at -20℃ to -80℃, valid for one year. Once reconstituted, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2-8°C for 2-7 days. It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing. Note 1. Avoid repeated freezing and thawing. 2. For your safety and health, please wear a lab coat and disposable gloves when operating. 3. This product is for scientific research purposes only. Product Data
