Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. Within the ECD, human Siglec-10 is most closely related to Siglec-5 (42% aa sequence identity). It shares 63% aa sequence identity with mouse Siglec-G. Siglec-10 is expressed on eosinophils, neutrophils, monocytes, and B cells with some splice variants predominating in particular cell types and tissue locations. It is upregulated on eosinophils in mouse models of allergic respiratory inflammation. Specifications Synonyms SLG2, SIGLEC10, MGC126774, PRO940 Uniprot No. Q96LC7-1 Source HEK293 Cells-derived Human Siglec-10 Protein, Met17-Thr546 with hFc tag and Avi tag at the C-terminal Molecular Weight Approximately 85.8 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Activity ELISA Data: Immobilized Human Siglec-10 at 2 μg/mL (100 μL/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.4 ng/mL determined by ELISA. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage Transport with ice packs. Store at -20℃ to -80℃, valid for one year. Once reconstituted, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2-8°C for 2-7 days. It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing. Note 1. Avoid repeated freezing and thawing. 2. For your safety and health, please wear a lab coat and disposable gloves when operating. 3. This product is for scientific research purposes only. Product Data
