Matrix metalloproteinases are a family of zinc- and calcium-dependent endopeptidases that have the combined ability to degrade all components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a variety of substrates, including gelatin, type IV and V collagen, elastin, and proteoglycan core proteins. It works in concert with interstitial collagenase (MMP-1) to degrade fibrillar collagen when it degrades its denatured gelatin form. MMP-9 is produced by keratinocytes, monocytes, macrophages, and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Specifications Synonyms MMP-9; GELB; CLG4B; MANDP2; Gelatinase B Uniprot No. P14780 Source HEK293 Cells Sequence Ala20-Asp707 Tag C-His-Avi Molecular Weight The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Activity ELISA Data: Immobilized Human MMP-9, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Storage Store at -25~-15℃, valid for 1 year after receipt. After reconstitution, store at -85~-65℃, valid for 3 months Reconstitution method Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.。 Note 1. For your safety and health, please wear a lab coat and disposable gloves when operating. 2. This product is for scientific research purposes only. Product Data Tris-Bis PAGE Human MMP-9 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. The purity of Human MMP-9 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human MMP-9, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA.
