TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. TREM-2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes. It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria. In the CNS, TREM-2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination. TREM-2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts. Mutations in TREM-2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts. Soluble TREM-2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM-2 blockade exacerbates disease symptoms in the experimental EAE model of MS. Specifications Synonyms PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c Accession Q9NZC2.1 Source Recombinant TREM2 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains His19-Ser174. Molecular Weight Approximately 18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE. Activity ELISA Data：Immobilized Human TREM2, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-TREM-2 Antibody, hFc Tag with the EC50 of 28.6ng/ml determined by ELISA. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage Transported with ice packs. Store at -20℃ to -80℃, valid for one year. After reconstitution, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2 to 8°C for 2 to 7 days.。 It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing. Note 1. Avoid repeated freezing and thawing. 2. For your safety and health, please wear a lab coat and disposable gloves when operating. 3. This product is for scientific research purposes only. Product Data
