The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction.Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. Specifications Synonyms U-PAR; uPAR; CD87; PLAUR; MO3; UPAR Uniprot No. Q03405-1 Source Recombinant Human uPAR/PLAUR Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu23-Gly305. Molecular Weight The protein has a predicted MW of 34.36 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended . Dissolve the lyophilized protein in distilled water. Storage The products are shipped with dry ice and can be stored at -25~-15℃ for 1 year. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. Note 1. For your safety and health, please wear lab coats and disposable gloves for operation. 2. For research use only! Product Data
