Specifications Synonyms CCN2; IBP-8;IGFBP8; HCS24 Source Biotinylated Human CTGF/CCN2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln27-Ala349.[Accession | Q5M8T4-1] Molecular Weight The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Biotinylated Human CTGF, His-Avi Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CTGF Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only.
