Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Under reducing conditions, secreted monomeric Ang-2 is 65-66 kDa in size. Under nonreducing conditions, both natural and recombinant Ang-2 form 140 kDa dimers, 200 kDa trimers, and 250-300 kDa tetramers and pentamers. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus . It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel-Palade bodies. Both Ang-2 and the related Angiopoietin-1 (Ang-1) are ligands for the receptor tyrosine kinase Tie-2. Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang-1 during lymphatic vessel development. It mediates the upregulation of ICAM1 and VCAM1 on EC, which facilitates the adhesion of leukocytes during inflammation. Ang-2 is upregulated in both the endothelium and tumor cells of several cancers as well as in ischemic tissue. Its direct interaction with Integrins promotes tumor cell invasion. Ang-2 also promotes the neuronal differentiation and migration of subventricular zone progenitor cells. Specifications Synonyms AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand Uniprot No. O15123.1 Source Recombinant Biotinylated Human ANGPT2/Angiopoietin-2 Protein is expressed from HEK293 Cells with His tag and Avi tag at the N-terminal. It contains Lys275-Phe496. Molecular Weight Approximately 28.4 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGE. Activity ELISA Data: Immobilized Biotinylated Human ANGPT2, His Tag at 5μg/ml (100μl/Well) on the Streptavidin Procated plate. Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. Source < 1.0 EU per 1μg of the protein by the LAL method. preparation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. Storage Transported with ice packs. Store at -20℃ to -80℃, valid for one year. After reconstitution, store at -20 to -80°C in unopened state for 3-6 months. After reconstitution, store at 2-8°C for 2-7 days. It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing. Note 1. Avoid repeated freezing and thawing. 2. For your safety and health, please wear a lab coat and disposable gloves when operating. 3. This product is for scientific research purposes only. Product Data
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