Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein, His-Avi Tag

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Synonyms ITGA5&ITGB1; VLA-5;Integrin alpha 5 beta 1; α5β1 Source Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag a t the C-Terminus. It contains Phe42-Tyr995(ITGA5) acidic tail and Gln21-Asp728(ITGB1) basic tail.[Accession | P08648(ITGA5)&P05556-1(ITGB1)] Molecular Weight The protein has a predicted MW of 111.8 kDa (ITGA5)&83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 100-140 kDa based on SDS-PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human ITGA5&ITGB1, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-ITGA5&ITGB1 Antibody, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from the date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only. Show more