Product Properties Synonyms ETGF; TGF-alpha; TGF type 1; TGFA Species Human Source HEK293 Sequence Val40-Ala89 Accession P01135-1 Tag N-hFc Molecular Weight The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by Bis-Tris PAGE and HPLC Activity ELISA Data: Immobilized Human TGF-alpha, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA SPR Data: Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 μM as determined in SPR assay. Formulation Lyophilized from 0.22μm filtered solution in 50mM Tris,100mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. Appearance lyophilized powder StorageStore at -25°C to -15°C. The product is valid for 1 year upon receipt. After reconstitution, store at -85°C to -65°C and use within 3 months. Reconstitution Centrifuge before opening the vial. It is recommended to dissolve the lyophilized protein in distilled water. The reconstitution concentration should exceed 100 μg/ml. Note 1.Please operate with lab coats and disposable gloves, for your safety. 2.This product is for research use only.
